LOCUS       CO961165                 555 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18c11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q93456 Q93456 F10G8.7 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961165
VERSION     CO961165.1  GI:51334349
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 556
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gatcaaccgt taaagcagca aatattaatt gtctaacttc gacatcgcta aattcgaacc
       61 cctttggttc gtcgaaactg aaaataaaca gacgcagaca ggagggaaat cctttgctta
      121 aatatattcg caacgtggtt catgaatggg cagacataaa gtgtgatttc gaatgctcta
      181 aaaactgtgg cctcctgtat ctctcgctca agtatcataa attacactcg ggttaccttg
      241 agacgcggct tcccgatttg aaagattatc aagtcaagat actcctcgca tacgtgagtg
      301 ttgaggatcc atcttttctg ctccgtgacc tgaatatggt ctgctaccgt atgaacattt
      361 cacttgtgct ctgctactct gtggaagagg cagccgaata tattgagacg ttcaaaagga
      421 cagaaaacaa aaacgtggaa accgagttgt caaacattca acaataccgg ttaaagcgac
      481 aacaacagtt gcaattgaat aaaacctgaa ctatccatac aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa acaaa
//