LOCUS       CO961163                 754 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18c08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL19_CAEEL O02639 PROBABLE 60S RIBOSOMAL PROTEIN
            L19. [1], mRNA sequence.
ACCESSION   CO961163
VERSION     CO961163.1  GI:51334347
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 911
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aatgagcaat cttcgtctgc agaaacgact ggcagcttca gtgttgaagt gcggcaaggg
       61 caagatatgg cttgacccga acgaggtgaa cgagatttcc aacgcaaact cgcgccagaa
      121 catccgccga ctcgtgaagg atgggctcat cattcgcaag ccaggaaaag tacattctcg
      181 tttccgcgca cgtgaacgct tggaggcccg tcgaaaggga cgtcacatgg gtttcggtaa
      241 gcgccgtggt acgcgcaatg ctcgtatgcc ggagaaggtg ctctggatgc gtcgaatgcg
      301 agtgcttcgc catttgttga agcgctaccg ggacgccaag aagattgaca agcatctcta
      361 ccacgagctc tatctgaagg ccaagggaaa cgcgttcaag aacaagcgca acttgatgga
      421 gtacatcttt aagaagaaga ccgagaacca gcgctccaag caactcgctg aacaggcaga
      481 agcccgtcgc aacaagaaca aagagacgcg caagcgtcgc gaggaccgcc tgatccagaa
      541 gcgaaaggag ttgctccaca agatttcgga gagcgaaaaa gagaagacgc cacagccgat
      601 cgctgctcct gagcagccta agcagaagga ggaagttgca aagaaggcga ccaaggaagc
      661 aaaacaaccg accccgcaaa aggaagttgc cgcgccaccg aagaagcagg aggagaaggc
      721 gaaggaacag ccagcagcca aacagcccca gcag
//