LOCUS       CO961162                 483 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18c07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS16_CAEEL Q22054 PROBABLE 40S RIBOSOMAL PROTEIN
            S16. [1], mRNA sequence.
ACCESSION   CO961162
VERSION     CO961162.1  GI:51334346
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 582
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcttttgaa agaaaggcaa agggctgatc aaggtgaatg gacgtccgct ggagtacctt
       61 cagccggaaa tcctgcgcat caagctccag gagccgctgc tcgttgtcgg caaagacaag
      121 tatgcaggga ttgacatccg tattcgcgtg aaaggcggtg gacacgttgc ccaaatctac
      181 gctatccgac aggccttggc caagtcgctc gttgctttct accaaaagaa tgtggatgag
      241 cagagcaaga aagaattgaa ggaacagttc gtcgcctacg atcgcaattt gcttgttgcc
      301 gatccgcgtc gtcatgagtc taagaaattc ggcggacccg gcgcccgtgc tcgctaccaa
      361 aagtcatacc gttaagtgta tgcagatgat caaaatgcat cgatggctcg aatgttgtca
      421 accatttgtt tctgttatgt tggaaaataa acattattac aaaaaaaaaa aaaaaaaaac
      481 aaa
//