LOCUS       CO961157                 592 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18b12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O88547 O88547 COP9 COMPLEX SUBUNIT 7B. ;contains
            element MER22 repetitive element, mRNA sequence.
ACCESSION   CO961157
VERSION     CO961157.1  GI:51334341
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gctgctgaaa aagacacaaa aatttggtgt cattggcctc taaacacaac atattgcctt
       61 acgacctctt aatgggcgca ctcggaatca acaatcaaag agaattcgag gactacatca
      121 tccaatccat ttatgacaat gtaattcagg ggagacttaa tccaatgaaa ctccaactcg
      181 aactaagtaa ttggagtagt gaggggctta atgaaccgga acttaaattt atggcaacaa
      241 ctttgaaaca ctggtacaaa cacagtgatc aattcttgca cacccttgcg aaggaagtcg
      301 agcgttccaa aaaatgtgtc gcggaaattg tcgcgaatga aaagaatatt gcggatgaag
      361 ttgagaatag acggaaatca gtagctctgg acgaagccaa gaagttcttt ggggacaagg
      421 aaggcttcaa accgacgtct aagccggaga caaagcgctt taagagtggt gttcgaatga
      481 agcgcggtgg agactgaggc cacggtttcc gaagaagaaa ctaaatcgta aatcgacaag
      541 tttgaacaac atgtatttaa tgttttcccg aaaatcaaga tttggattac tt
//