LOCUS       CO961155                 729 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18b08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RT31_YEAST P19955 40S RIBOSOMAL PROTEIN YMR-31,
            MITOCHONDRIAL PRECURSOR, mRNA sequence.
ACCESSION   CO961155
VERSION     CO961155.1  GI:51334339
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 870
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gttgatgcgc aaatgctcaa gatgattcaa acattcatgc tcgacactgc actgttccgt
       61 ctgatcggac gcctcttctt cgcaacaatc ggcggcggtt ggccgagtcg aatgcgccga
      121 gccggcggcg agaagaagcc accgctgcgc caggcgagtc catcagcacg cgcaattttg
      181 cgcacgccgc actcagcaca gtccagttct ccattcagcc ggcgcgcaag ccaatgatca
      241 aattcctcgg acggcgccat ccgcagccca agccgttcga cccgaatgcc ggtggcgcat
      301 ccagtacagc ggcttcttcc aatgcaacaa gctcgtcaaa gaagagtgaa agcggcgcag
      361 aagtggtcga tgtgctggac ttccaagagc tgcccgcaat gttccggcgc gctccaatca
      421 gcgaagagga gatccaagtg atcaatagcg gcggattgcg caactgattc agtgacgcca
      481 aaagctccgt agagaaccca actctgattg actttgacgc gactagattt gtccaatcca
      541 aaggcacaca gacccaaata gagaggcccc cacgacccga ctcattgttt tgcttctccc
      601 atagctttag tcaaatcacc ctgtttcaca tttgctccac attcagtgag ctcaattcgc
      661 tgctgtccta ttatgtttga tgaatctttt caactttcat tcccgtccat tcttcataat
      721 cattatcgt
//