LOCUS       CO961147                 536 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18a09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:H4_CAEEL P02306 HISTONE H4. [1], mRNA sequence.
ACCESSION   CO961147
VERSION     CO961147.1  GI:51334331
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 562
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctaatactaa aacctaaaac gcaatgtcaa gtcggggaaa gggtggcaaa ggactgggaa
       61 agggaggcgc caagcgccat cgcaaggtgt tgcgcgacaa catccagggc atcacgaagc
      121 cggcaattcg ccgactggcg cgccgtggcg gagtgaagcg cacctcggga ctgatctacg
      181 aggagactcg ctctgtgctc aaggtgttcc tcgagaacgt catccgcgac gcggtgacct
      241 attgtgagca cgccaagcgc aagacggtga ccgcaatgga cgtggtgtac gcgctgaagc
      301 gccagggccg caccctgtac ggatttggag gctgagcaga ggacaccaaa tgggaggtcc
      361 caccatccct tggatccaac tatgtatttt ttcttgactt cctccacttg tctcctgtcc
      421 aatgtttgat tttcgctgcg ctctggttat tttgagtacc gatgataaac cgatccgaag
      481 acacagaaaa aaaaaaaaga aaaaaaaaaa atgaaaaaaa taaaaaaaaa tacaaa
//