LOCUS       CO961146                 517 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe18a07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q21955 Q21955 SIMILAR TO NOTCH PROTEIN IN AND JUST
            UPSTREAM OF THE POLY-GLN REGION, mRNA sequence.
ACCESSION   CO961146
VERSION     CO961146.1  GI:51334330
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 520
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctcgtcttgg acacttctgg ccctgacccg accccggttg gccctcccag cgctaaagtc
       61 cccagatggg cgaccctccg cacccggcga atggtgctcc cgcttgtccc tggcaggagt
      121 tccgggacga cgtggtcagc cgcctagagc tggtgctgtc gcagaaccag cagaacatcc
      181 acaagctgcc gctgcccaac gacgcacggg agattgagaa gtcgctgtac ctgaggtacg
      241 aggacaagga cgagtacgcg agggccatct ccaaggtgat actggcgatc aagtgcaaca
      301 ctgcggagag cgccatcaca gcggacgtgg acctgttcgt gcagaaggtg ggcgccttcc
      361 tgcgccagct gcccgccgac agattccagg aggccagggc ccagatggac cagctgatgg
      421 ccgcatttgg tgcctccgag aaatgacacg ggcgacccgg ggacggacga ttcgatgttt
      481 tctttcaaat aaaaagttaa cgcctcaaaa aaaccaa
//