LOCUS       CO961142                 720 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17h12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains MER22.t1 MER22 repetitive element, mRNA
            sequence.
ACCESSION   CO961142
VERSION     CO961142.1  GI:51334326
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cggggcgcat cgacatccat cgactgttcc agttggacag cattcagcag ctgcaggcgc
       61 aaaaaagggc gcacaacaag cagaaacagg ccgagaaggg ggccgtggaa gcggagggaa
      121 cggacatgga ggtggaccag tgatgagccc caaacgaccc aagtgaccga acagatcctc
      181 atttcagtcc aataatgtaa tttgttacac gctttgatcg aggttttttt gactggacca
      241 gtaaaggcat tctttaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      301 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaccaaaacc ccaaaactat
      361 ttccccccca gggggaaatt cggcaaaaac cctccacccg gggggccccc caaccaggct
      421 ttaaaggggc ccatttcccc caaaagggat tctaattaaa tttcccgggc ccccttttta
      481 aaacttcggg ccgggaaaac cccgggcttt ccccacttta ttccccttgc accacacccc
      541 cttttccccc ccggggtaaa aaccaaaaag ccccccccca ttcccccttc ccaaaatttc
      601 cccccccgaa agggcaaagg gcccccccct gaaccgcccc tttacccccg ggggggtggg
      661 gggttacccc ccccttaccc cctccctttg ccacccccct accccccccc cttttccttt
//