LOCUS       CO961132                 592 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17g04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q21930 Q21930 R11D1.8 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961132
VERSION     CO961132.1  GI:51334316
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 596
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tctactcaac aaggagaaga gagatataat acagccatgt cgcgcatcaa caacgcatct
       61 agctacattc agtggcaagt gatccgcaag aacagcgcgt tcatcaagcg ccaacgcggc
      121 attccgaagc agttcagcac agaaccgttc aacatggccc gtgtgaacag catccgccac
      181 aacggaatga tcaaccggaa ggcggtggac gtacagccgg ccaagggcgg gaagggagtc
      241 actgtgacca tcaagaagaa gaggaactcc acccagcccg caaagtcgac ggtggtgacc
      301 acgctgagca aggacagccg tgcagtgacc gcctcgatcg gaaatgcgat cggcacgtac
      361 aagtcctctc aggcgaaact ggcccagcgt cgcgcgtcgc agatcctacg ctcacaaaaa
      421 gtgaaggcaa agccggtggc agcgaagaag ggcaaaactg agtaaaagaa tgagagatgc
      481 aaggggagaa ccgactgaat gattccggag aggaattttg ttgttgttgt tggatgaaat
      541 gttaagtaaa ttcatttctc ccacggaaaa aaaaaaaaaa aaaaaaaaca aa
//