LOCUS       CO961129                 468 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17g01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9VWD1 Q9VWD1 CG14235 PROTEIN, mRNA sequence.
ACCESSION   CO961129
VERSION     CO961129.1  GI:51334313
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 555
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 caaggacgaa aatgaaatcc atatgatgca cccggacaac ccgaaccaat ggaatcccgc
       61 acttcacgag aaagagagca aggagttttc ttgggccgcc ccatatgatg ctcgttttcc
      121 gcaaacccgc aagcaacgcc attgtttcgc ctactatgtc gactattttc gctgtctgga
      181 gttgatggga gaggattaca agccgtgcaa attctttcag aacgtctacc gagacatatg
      241 cccgggtttt tgggttgaga agtgggacga atttcgcgaa gagggacgct tcccggcaaa
      301 gtttgatcgc tgagcacttg actccttaca gcataggaag aaaccaaatt caattcacac
      361 attattcaaa ggaatagagt taaacgatgc gttacttttt gtgacatccg attgcgtaaa
      421 ttttgggttc aaaaaaaaaa aaaagaaaaa aaaaaaaaaa aaaacaaa
//