LOCUS       CO961119                 449 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17e12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL34_SCHPO O42846 60S RIBOSOMAL PROTEIN L34. [1],
            mRNA sequence.
ACCESSION   CO961119
VERSION     CO961119.1  GI:51334303
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 454
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttagaaaatg ccgcagcgtt tgacctatcg caggcgactc tgctacaaca caaagtcgaa
       61 caagaccaag gtggtcaaga caccaggagg ccgcaatgtg ttacactatc gcaataagct
      121 cggctccgtg cccaagtgtg gcgacactgg cgtcccgctg aaaggaatcc aagctgcgcg
      181 acctcgccaa ttggccaagc tgactaagcg aaagaagacc gtcacgcgtg catacggagg
      241 atgcctgagc gctgcagcag tgcgccagcg aataatccgc gcattcttgg tcgaggaaca
      301 gcagattgcg aacaagatcc tgaaagccaa gagtgctgga aggaagtaaa ccgatggaag
      361 gttttggttc caccatgttc tatttgttgt tgcattaaag tttgttctgt ccatcctgga
      421 taaaatcacc agttgctaaa aaaaacaaa
//