LOCUS       CO961114                 607 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17e07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL21_CAEEL P34334 PROBABLE 60S RIBOSOMAL PROTEIN
            L21. [1], mRNA sequence.
ACCESSION   CO961114
VERSION     CO961114.1  GI:51334298
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gagacacacc tgttctgaag caaatggtca accccaaagg ataccgccgt ggcacccgct
       61 acatgttcgc gaggccgttc cgcaagcacg gcgtggagcc gctgtccacc ttctaccggg
      121 tgtacaagcg tggcgacatc gtcacggtca agggcaacgg cgccttccaa aagggcatgc
      181 cgttcaaggc ctaccacgga aagactggcc gagtgttcaa cgtgaccaag catgcgctcg
      241 gcgttgtggt caacaaacgt gttcgcaatc gcatcatccc gaagcgaatt aatgttcgcg
      301 tggagcacgt caagccatct ggctcccgct cggagttctt gcgccggtgc aaggccaacg
      361 accagcagaa gaaggaccat aaggccggaa atgcgccata tgttccgctg cgccgcaaac
      421 cgcaggaacc gagaggcgct catctggtca agctgaacac gacgcacatc gaagagctgg
      481 cgccagtgaa gttcgaaatt gttgcttgag tgagaccatg tttcggtgtt cgcaacacac
      541 tatgttattg ttgacttgat cgtaaaaata tgaaaatgat caaaaaaaaa aaaaaaaaaa
      601 aaaaaaa
//