LOCUS       CO961107                 526 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17d09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961107
VERSION     CO961107.1  GI:51334291
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 542
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcacgagagc gaacttggac gagtcgagcg gcgcccatct cgactgcacg gcgaatggcg
       61 ttcatcgcat ggaacagcac ccccgaggag gtggatgcgc ccgccgaagc ggaggaaccg
      121 aacaacaatg gaacaatgca aactgggagc agcgctgagg aagaagtgaa gagcgccatt
      181 cggccaacgg acggaacaac aacaacagag caagaaggaa acggacgaac aacaaaaaca
      241 aagcacagcc gcagagtctt tctcgacgca ttcggcaccc ccagcagagt ctttcacaag
      301 ccattcattt tcggtctttc atccccgcaa tccaccattt gttgcccgca ccccaaaagc
      361 ccaaaaacag aactacaaac acacacaaac acaattgtcg cggtcagcac tgtgcccgga
      421 ggactgtgac caaaacatgc cctaaatgtg cgaatgaata aaacggattc accacaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa accaaa
//