LOCUS       CO961101                 652 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17d01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961101
VERSION     CO961101.1  GI:51334285
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gacgcattgg tggagtccac tctaagtttc gccggctctc tctcaccgga agctgttggc
       61 gcgttcttca cgttcgtgga ccactcgcgc gcgttcagcc actgtgctcc cgcattgagc
      121 aagtgcgcgg gtttcctgga ggccaaaggc gactcccttc acgcccattc gctctaccgg
      181 cgcgcgctgg aactggtgcc gggacacgag ccagcaacga gttgccacgc caaattgttg
      241 cctttcgtgg aggaacacga caccaaggag ctgaacgagg cgctcgacaa gaagaggcga
      301 cttcgcgagt tgttgtcaaa gcagctgaaa acggacgagg gagtctcggc acttcgaaag
      361 gcattctgca ctcagctctt ccacacctgt cgcatggagg gtaactcgtt gagctctgcg
      421 caagtgctcg agacggttac tgcgggagaa cagcagcagg acgaggcgga cgacgagccg
      481 ccacacaaga gagcacgtgc ttcatccgca tccacatcgt tgtcgggagc tgccaaatcg
      541 ttgattcggg agcagttggc cgaccagtcc cagaaggcgg tgggccaacc ggtggagcag
      601 ttagccatga aagcacgtgc ttcatccgca tccgcatcgt tgtccggagc tg
//