LOCUS       CO961096                 566 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17c07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961096
VERSION     CO961096.1  GI:51334280
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 636
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggctattgat gaccttcagt cagcgaaaat atcgcgccaa tcgccacagc caacagccag
       61 tgccccttcc attgcaattg atcgatcacc gctctcggtc ggaatcactg ctctgagact
      121 ggatgaagcc tctctgtgtc tccgcactct tcttcttctt cctcctcctc ctcttcgctt
      181 caatgctctt ctgcgccaaa gcggatccac agaaggtgtg caggtcggtt gtcggattcg
      241 gtggggaaca gggcaaaatg gagcgctgca aacgctgcga gtacaaacgg tgcggcatcg
      301 agtgcgagct gcccgaccag aagtgctacg actgcgtgta caacaactgc cgcggatgaa
      361 aactaaaggg ccgctggaac agtgcaaatg cggatggctc cctttgctgt tccaaagaat
      421 tctttgcatt ccgttctgcg tttgttcaag caaaaatccg atccttttcc gcctcgtttt
      481 ctcggacaaa acgcttctct ttctttcgaa atacacatgt cataagtcat aactcataac
      541 tcaccaaagt gggccgccat aaagcc
//