LOCUS       CO961095                 362 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17c06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O48829 O48829 PUTATIVE GLYCINE-RICH, ZINC-FINGER
            DNA-BINDING PROTEIN, mRNA sequence.
ACCESSION   CO961095
VERSION     CO961095.1  GI:51334279
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 673
            Seq primer: SL1 primer
            High quality sequence stop: 360.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggatcgaata gctaccgatg ccatgagatg ggccacatct cccgcgagtg tcccaactct
       61 ggtggtcaag gaggtgggcg tggtggtgga ggaccacggg gaggcggtgg agatgtgaaa
      121 tgctacaatt gtaacggcta tggacatttc gctcgcgatt gtcgtgatgg tggtggagac
      181 cgccgtggcg ggggcggtgg tggtggacga ggcaatgacc gcggcactaa gtgctacaac
      241 tgcggagggt atggtcactt tgcgcgcgaa tgccgtgatg ggggtggaga ccgccgtgga
      301 ggaggcggac gtgacaaccg tggcgatgat cgccgaggag gcggtggagg tggcggccgt
      361 ga
//