LOCUS       CO961090                 755 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17b11.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q21581 Q21581 COSMID M60. [1], mRNA sequence.
ACCESSION   CO961090
VERSION     CO961090.1  GI:51334274
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 768
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gttaacttca ataaaaatgg cgatgaacct caacactggc tacctgagta ccaaccgtgg
       61 tattatcaaa atttctgcag agtcatctgc ggtttcatca cttgctccca gctttgagcc
      121 aacaggtatg gcggcaaatc ctagcttcta tgacggccga gtcggctacg caagaggact
      181 caacttcgtc atcgtcatca tcaacattgt cctctttttc ctgaacttcc tgaatgttgc
      241 ggtgtaccgc atggaacgcc tctattcggt ggtgggcgtc atcctgttca tcatcgcgtt
      301 cggagtgatg atctggtact tgattgagta tgaagtgaac cgtgggcagt tcattgctgc
      361 gacggtcttc atcgggatac agatggtcct gttcatctgg gattaccaaa ttctgcacgg
      421 acaggcatac aactgaggag acgaggatgg accccaaact gggaaatgtt tttggatcgt
      481 gtaatgtacc gtcgcaacaa ccaacctata tatcccctct ccaattcttc ccatcctacg
      541 cttattctca ttatcagcta aacttaagca cctctaacca atcacaccaa acaattttgg
      601 ctttttggca tccgactgcc agtctctctc tctctctttg catcgtccaa agtccaattt
      661 tccaaaatat ttgtaactgt ccctttcttg ctgacaaatt aaatctgtac tagtaaaaaa
      721 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aacca
//