LOCUS       CO961089                 716 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17b10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961089
VERSION     CO961089.1  GI:51334273
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 724
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cgatgagcga acacaaaaaa ccgaacaatc cggccaaccg caacagaatc agaaaggcgg
       61 ctactacgag cgagcgaaag acactttggg ctctgtaaaa gattcgatct ctaataccat
      121 tgagtctgcg aaggagaaag tgtcggaaac cctgggcaca gcaaaagaga aaactgccga
      181 cacttacgaa agcggaaagg agaagattga agaggcaggc cgcgaatatg atgaagcaaa
      241 ggagaaggcc ggcgagaagt tccaggcggg aaaagaggcg acagccgatc gaatgtacag
      301 ggctgcagac aacatcagag gaaagtgaac gccggagtct gagtccttgc aattgtattg
      361 gctgttttct cctggattct cgtcttcgtc ttacttcagc cggttaatca ttaataacca
      421 attctgtatt aatatttttg tctcttacca cgcttgaatg tcacacagat gccaatagtc
      481 caaaatttag caatattttt taatcaccac aagcagaaag cacactcact cagttttcat
      541 ttttaattaa tacaccacac caccatctac taaaggaagg tacaacgaaa caacattaat
      601 aaaaccaaaa tcacattatt tgtcaatgaa tttttgcata aattatgcat gtttttattc
      661 gtcttattga taaaagttaa tccgcacaaa aaaaaaaaaa aaaaaaaaaa aacaaa
//