LOCUS       CO961082                 485 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17a09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:DYL1_CAEEL Q22799 PROBABLE DYNEIN LIGHT CHAIN 1,
            CYTOPLASMIC. [1], mRNA sequence.
ACCESSION   CO961082
VERSION     CO961082.1  GI:51334266
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 488
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cttctcgcta tataaaaagc atcaatgggg tgaccgaaaa gccgtaatca agaatgctga
       61 tatgacagaa gagatgcaac aagaggcgat cgagtgcgcg actcaagccc tggagaagta
      121 caacatagag aaggatattg ctgcatacat caaaaaggag tttgacaaaa aattcaatcc
      181 gacttggcac tgcattgttg gccgaaactt tggctcctat gtcacgcacg agacgaagca
      241 cttcatatac ttctacttgg gccaggttgc cattctcctg ttcaagagcg gctgaacgtg
      301 aaatatgttc aatgaagaca aatcagatca gataaactgt gttttcgact gacgcgttct
      361 atctacgttt aactcattaa aacctatttg ttttgtctac atggaataaa agctctggcc
      421 ggcaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      481 ccaaa
//