LOCUS       CO961081                 424 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17a07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q13579 Q13579 MARINER TRANSPOSASE, mRNA sequence.
ACCESSION   CO961081
VERSION     CO961081.1  GI:51334265
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 430
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcgaacccta atattgtacg aattcgacca aggtcataca acaacgcagg cgcatcgaaa
       61 catcaatgaa acaaagggcc cagggactgt ctccaaagcc acagtgaaga gatggttcgc
      121 gcagttcaga aatggcgaga acatcctcaa ggacaaaccg aggtctggaa ggccatcgac
      181 aatcgatgag gacgatgttc tggctgctgt cgaagctgat ccgaccctga gcacgcgcat
      241 gttggccgtc gacttccact gttcgcacat gcaaatcgcc agaattttgc acaaactcgg
      301 gaaggaagta cggaagggaa gatgggtccc gcatgatctg accgtcaacc aaaaaaacca
      361 gcgtttttta ataaataatt tattttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaac
      421 caaa
//