LOCUS       CO961080                 633 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17a06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q99633 Q99633 HPRP18, mRNA sequence.
ACCESSION   CO961080
VERSION     CO961080.1  GI:51334264
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 508.
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aagagggccg atttgttcaa gaaggagcga gaggcctatc tggagaagca acggcagttg
       61 gagctggaac agcagcagaa tgtgaaggga cagagtgtgg acgatttgct ggacgcgaag
      121 ctgtccgccg attccatgga ctcgattccg cgagcggagg tgatccgacg cctccgcgcc
      181 agaggtcagc ccatcctgct gttcggcgag accgaagtgc tggcactgag gcggctcaga
      241 cgactggaga ttgagagacc ggacctgaac gagggctgga aaaacgactt ccaggcggcc
      301 ctgagccaag tggagaagga ggaggtgatg gaacaagtga tcaagggcac ccgagacaga
      361 gacgaggcgg gcagacacga cgtccaggtg cagganagcg aagacgacgc aacttgggag
      421 caaatcgtga gtcgagctcc aaagctgggc agtggagacg agcagtatga ctggtgactg
      481 atcttggcct acatcaacta catcctgaag aagtggggca agggagctaa ctcgcgcgag
      541 gagagcgtga ngcgcagcgt gcggggcnaa ctgcaagctg gaatgcatac gcagaccgtg
      601 gaccacttgg ctcagttgat cactcgcacg aga
//