LOCUS       CO961079                 440 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe17a05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P90836 P90836 F13G3.10 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO961079
VERSION     CO961079.1  GI:51334263
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 460
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggaggcacag agtgaggacg ccaagcagga taagcaccgt tgggagcggg atgcggccga
       61 tttggagaag gtcactgatt ttcacgagga gaaggatgaa atgaacgaga tttcgaagga
      121 gtcgctgggc aacttgaagg cacagtcggg cgccaaaaag tttacggtta agaaagagga
      181 cgtccaaaca attgtccaag agttggaggt gccaaggata attgccgagc gtaaactcgt
      241 ccaacacgag gggaatttgc gagcagcgct aatggacttg attggattcg agtgagcgga
      301 atgccgaata ctgctccgca gaaaattgtt cagtacttat ttattgtctt cctggttctc
      361 tgatatcggt ggtgttacgc acgtcgatac tgtttgcgaa taaaacctgt caaatgaaaa
      421 aaaaaaaaaa aaaacaaaaa
//