LOCUS       CO961072                 498 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16h05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:R13A_CAEEL Q27389 PROBABLE 60S RIBOSOMAL PROTEIN
            L13A. [1], mRNA sequence.
ACCESSION   CO961072
VERSION     CO961072.1  GI:51334256
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 669
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgaagcaatc aacatctccg ggaactttca ccgttctaag ctcaagttcc ttgcatttct
       61 gcgcaagcgt tgcaatgtga agccatcccg tggtccattc cattaccggg caccgtcgcg
      121 tatcttctgg cgcacggttc gtggcatggt ccctcacaaa actgctcgtg ggatggaagc
      181 gctgaagaaa ctgaaatgtt ttgacggctg tccccagccc tacgacacga agaagaaatt
      241 cgttctaccc aactctcttc gccacatcgc actcaagccg cgtcgcaaat gctgcactgt
      301 tggacgtttg gcgcacgagg tcggttggca gtaccaaggc gtcgtggaga aactggaggc
      361 caagcgcaaa gtgcgtggtg ccgccttcca tgagaagaag aaggccgagc agaagctgaa
      421 gaagcaggca ttggagaaat caaaatccaa gactgccccg ttcgacaaga ttttggcgca
      481 gtacggatac gcctaaat
//