LOCUS       CO961071                 664 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16h03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL7A_FUGRU O57592 60S RIBOSOMAL PROTEIN L7A, mRNA
            sequence.
ACCESSION   CO961071
VERSION     CO961071.1  GI:51334255
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 455.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 aaagaacacg cttttcgaga aaagaactcg caacttcgca ataggccagg acatcattcc
       61 gaagcgggac ctgactcgct ttgttcgatg gcccaagtac atccttctcc agcgccagct
      121 ggccgttctg atgaagcgtc tcaagatccc gccgaccatc aaccagttca actttgcatt
      181 ggacaagccc aacagtgcgc aactgcttaa gctgctcaac aagtaccgac cggagacgcg
      241 tcaggagaag aaagaacgac tgcgccagcg tgcccaggac cgggtcgagg gcaaacaaga
      301 gaaggtgacc aagcgtccgc cggtggtcca cttcgggatc aaggacgtgg tcaagcaagt
      361 ggagaacaag aaggccagtc tcgtggcgat tgcacacgat gttgacccga tcgagatcgt
      421 catctgtctg ccggccgtct gccgcaagtt tggcgtgccc tatgtcattg tcaaggggaa
      481 ggctcgcctt ggacaagttg tgaaccgcaa gacctgttcc gctgtgtgcc tgtcagacgt
      541 gaacccggaa gacatgacag cactgagaac ttggccgaag tggctccacc aacttcacga
      601 gcgcgagatc agatcacaag cactgggcgc ngctctcgcn gcgaaatcca gccccaaggc
      661 taat
//