LOCUS       CO961068                 614 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16g10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q23683 Q23683 ZK970.7 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961068
VERSION     CO961068.1  GI:51334252
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 622
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcctttttc tccggcattt gccttttggc cctcttcgtg gcttgctcac tcgcccagca
       61 gggcgaagcc cctccaccgc cgttcctgga gggcgcgtcc gcagagaccg tgaaatcatt
      121 ccacgacttg ctgtccaacc tgggccactt gactgaccag cagattgacg acaaagtcaa
      181 ggaatgggtg ggccagcagt ccgaagacct caagaccaaa tatgccgcat ttgagatgga
      241 gaggcaaaag gcatcggaag ccggtgagca ggcacaccag gcagcagtgg ccaaattcag
      301 cgcagaggca aaggcggcgg acgacaagat cagcgcaatt gccaacaatc cggctttggc
      361 ggccaaggac aaggccgcac agattcaggc catcgtggag ggactgccgc anaatgtgcg
      421 cgacgagatg gagaaagcca tgggcattgg ggggcactga acaacgagca atcgagtgga
      481 aacgaaaaac accggttgat tcaatgacac tcgaagctga attgaatggc tttgcacaaa
      541 tcacagtaat aaacagtagt tcccaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag
      601 aaaaaaaaac caaa
//