LOCUS       CO961066                 495 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16g08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS9_RAT P29314 40S RIBOSOMAL PROTEIN S9, mRNA
            sequence.
ACCESSION   CO961066
VERSION     CO961066.1  GI:51334250
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 681
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tcagaaacaa gcgagaggtg tggcgtgtca aactgacgct ggccaagatc cgaaaggcgg
       61 cccgtgagct gctgactctc gaggacaagg accaacgacg tctgttcgaa ggcaatgcgc
      121 tgctccgtcg tctggtccga gttggcgtgc tgcccgagga caaaatgaag ctcgactacg
      181 tgcttggtct ccgcgtcgag gacttcttgg agcgtcgttt gcagacacag gtgttcaaac
      241 tcggactcgc caagtccatc caccacgcac gtgtcctcat tcgccaaaag cacattcgtg
      301 tgcgtcgaca ggtcgtgaac atcccatcgt tcattgtgcg tctggactcg cagaagcaca
      361 tcgacttttc gaccaagtcc ccgtacgcag gaggacgccc tggccgcgtc aagcgacgca
      421 gcatgaagaa gggcaccggc ggcggatctg gaggcgaaga agaggactaa gctcccccag
      481 aaagaccaat ttgag
//