LOCUS       CO961064                 454 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16g06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL10_CAEEL Q09533 60S RIBOSOMAL PROTEIN L10, mRNA
            sequence.
ACCESSION   CO961064
VERSION     CO961064.1  GI:51334248
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 721
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggccgaaaac gcgccaccgt cgacgaattt ccatgctgcg tgcatatgat ttccaacgag
       61 cgtgaaaatc tatcgtctga ggcgcttgaa gccgcacgta tttgtgcaaa caaatacatg
      121 gtcaagaatt gcggaaaaga tggcttccac atgcgtgtcc gaattcatcc gttccacgtg
      181 cttcgcatca acaaaatgtt atcatgcgcc ggcgctgacc gccttcagac ggggatgcga
      241 ggggcctttg gaaaaccgca agggtttgtg gcccgtgtcg gaatcggcga tgtgctgatg
      301 tcagtgcgtg tccgtgacca acacgaggct catgctctgg aggctttccg acgtgcaaag
      361 ttcaagttcc cggggcgcca gtacattgtc gtggtcgcgc aatggggctt cacgcaattc
      421 gaccgcgaag tgtacgagaa ataccgcaaa gaag
//