LOCUS       CO961063                 564 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16g05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RS12_PIG P46405 40S RIBOSOMAL PROTEIN S12, mRNA
            sequence.
ACCESSION   CO961063
VERSION     CO961063.1  GI:51334247
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 607
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ccgttttcca ccgaggaagg agcaatttca acaacttcac cagatactca taaaacaaaa
       61 tggctgagtc tcttgagatg aacgaggcgc tcaaacgcgt tctcaaatca gcgttgattg
      121 tggatggcgt tgcacgtgga ctcaacgagg ctgcccgcac tctggacaaa cgtgaggctc
      181 acttctgtgt gttcgccaag agctgcgatg aggcctcgta cgagaagctg gtgcgggccc
      241 tgtgcaagga gcacaacatc ccggtcatcg aggtggagga caaggcgcag ctgggccaaa
      301 tggttggcca atgcaagtac gacaaggacg gagctgctcg caaaatcgtc ggctgctcgg
      361 tagccgtggt caaggactat ggccgtgacg aggaggcgaa gaacgaactt caggagtaca
      421 tcaggaaggg tgtcaaggca gcggaataag cccgccgaga ccgtctcaag acgtcaagag
      481 ctctccacag tttgttgttt caatttatgt actgttgcaa gaattccaat aaattgttat
      541 ttgttaaaaa aaaaaaaaaa aaaa
//