LOCUS       CO961062                 410 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16g04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9Y4Z1 Q9Y4Z1 LSM3 PROTEIN, mRNA sequence.
ACCESSION   CO961062
VERSION     CO961062.1  GI:51334246
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 424
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 agtgcaaatg gccacaacta ctgctgattt aagcgtagcg gtagaggacc tacttgattt
       61 gatccggcta agtctcaatg aaagagtgta tgtgaagcta cgacaagatc gtgagctacg
      121 tgggcggctg cacgcattcg atcaacattt aaacatgata ttggctgatg ttgaagaaac
      181 tgtaaccatt acagagactg atgaggagtc ttttgaggag atttacaaat cgactaaacg
      241 acaaatcccg atgctgtttg tgcgcggaga tggcgtcatt ttggtttcgc ctccgaccaa
      301 atatattcct tgagtcttac cacgcagagt ctcatttgtt agactattgg tcaataaata
      361 tttgcgatca caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaccaaa
//