LOCUS       CO961060                 646 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16g02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961060
VERSION     CO961060.1  GI:51334244
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 685
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttctccgttc tccggtatgt ccccactcca caaaataact tgtggccgcc ctactggcca
       61 cagtgctcga atcaagccct ttgccctttg acggcaggca gtacccgtcc atcgacctga
      121 ggctcgtggg ctggccattg gcggacgaag gcgcagaatc gaggggcgac caacaggcga
      181 cgcggctgca gtgctacacg gagctggacg cgtcgtttgt ggacgggcag aggctgtacc
      241 ggctggagag cggcggccag ccggagggtc ggtcctgcct ggtgcgcgtg gcgaccggca
      301 ctcggctgac cctgtcccgg atgggcaccg ccagccctga cagctacgtg cgagtgctgg
      361 ccggctcatc gccgctgcac atgcctttgg gaacgcgacg aattcacaag ggccaactgg
      421 acttgtacgg ccccgggcag gtgccctgtg agtcggccta cctggtgttc agcacagggg
      481 actcttcgcc gcgggagtgg gtcgaattcc ggctggagag ggatacgcat gaggacgcca
      541 ggacgacccg gcagagggcg aaggaccttc aggggactga cccataaggg cccattggat
      601 acacccacat atgctcactt gtcctacgtt tggacaaaac tgttgc
//