LOCUS       CO961051                 738 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16f03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961051
VERSION     CO961051.1  GI:51334235
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 751
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gttctccgtt ctccggtatg tccccactcc acaaaataac ttttggccgc cctactggcc
       61 acagtgctcg aatcaagccc tttgcccttt gacggcaggc agtacccgtc catcgacctg
      121 aggctcgtgg gctggccatt ggcggacgaa ggcgcagaat cgaggggcga ccaacaggcg
      181 acgcggctgc agtgctacac ggagctggac gcgtcgtttg tggacgggca gaggctgtac
      241 cggctggaga gcggcggcca gccggagggt cggtcctgcc tggtgcgcgt ggcgaccggc
      301 actcggctga ccctgtcccg gatgggcacc gccagccctg acagctacgt gcgagtgctg
      361 gccggctcat cgccgctgca catgcctttg ggaacgcgac gaattcacaa gggccaactg
      421 gacttgtacg gcccctggca cgtgccctgt gagtcggcct acctggtgtt cagcacaggg
      481 gactcttcgc cgcgggagtg ggtcgaattc cggctggaga gggagacgca ggaggacgcc
      541 aggacgaccc ggcagagggc gggggagtgt tccgcagggg gagcggtcaa tcacgcggac
      601 caggaattcg aaggaccttc aggggactga cccaaagggg ccaattggag acacccacaa
      661 atgttcactt gtcctacgtt tggacaaaac tgttgcaaac ataaaactga accaaaaaaa
      721 aaaaaaaaaa aaaccaaa
//