LOCUS       CO961049                 560 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16f01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9TTK5 Q9TTK5 RIBOSOMAL PROTEIN S4Y ;contains
            element MER20 repetitive element, mRNA sequence.
ACCESSION   CO961049
VERSION     CO961049.1  GI:51334233
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 caagcttcgc gagtcgctcc cgctcattct ttttctgcga aaccgtctca agtacgccca
       61 gtcctataac gaggcgcgca tgatctgcaa gcaacgtcta attaaagtag acggcaaggt
      121 gcgaacggag atgcgtttcc cggctggatt catggatgta atctcgattg agaaaactgg
      181 cgagactttc cgtttgttgt acgatgtgaa aggccgcttc acagtccatc gcatccaaaa
      241 agtggaggga caatacaaat tgtgcaaggt ggtcaagcaa gcgatcggtc ccaagcaggt
      301 gccatacgtt gtgacccatg atgcacgcac cattcgctac ccggacccgc atctaagagt
      361 cgatgatacc gtggtgattg atattgtgag cggaaaaatt agcgactacg tgaagttcga
      421 ctccggcaat ctctgcatga ttaccggagg ccgcaacatg ggtcgagtgg gcatcatcgg
      481 acacagagaa cgccacccgg gatcgtttga cattgtgcac atcaaagact tggccggaac
      541 cagttcgcca ctcgtgtgaa
//