LOCUS       CO961048                 466 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16e12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O77095 O77095 HIGH MOBILITY GROUP PROTEIN 1.1. [2]
            TR:Q9U2A0, mRNA sequence.
ACCESSION   CO961048
VERSION     CO961048.1  GI:51334232
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gactcaatcg ctaacaacac aaagttcaag ccccaaaatg ccaaaggcct cctagaagaa
       61 caaggacccg aacatgccga agcgggcgca gagtgcctac ttcatctgga tgaacgcgaa
      121 ccgcgacaag atcaagaagc caggcatgag cgtcgctgag gtggccaagg ccgctggtgt
      181 tgagtggggc aaactgcctg cagctgagaa gtccgtttgg gagaagaagg cggctgacga
      241 caagaagcgc tacgagcagg agatggccgc ctaccgacag cagcactgag ctgttcgaag
      301 tggatgtgga cctgcagatc cactcgccgc cagaccatca tccaatcccc ttcttcgccc
      361 gtatataact cgtgtctcat ctatatatgc atgtgtttgt tttgaccttt tgacaacaaa
      421 gaaaaacaca ttcatctttt acaacggata aaatttaaat gaaccc
//