LOCUS       CO961046                 687 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16e10.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL11_CAEEL Q94300 PROBABLE 60S RIBOSOMAL PROTEIN
            L11. [1], mRNA sequence.
ACCESSION   CO961046
VERSION     CO961046.1  GI:51334230
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 690
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cttattggaa ttagcaacga tgacgaaagc agaacaagtt gccgataaaa aggagaagaa
       61 gttggcgaat ccgatgcgcg aactgaaaat ccaaaagctt tgtctgaaca tttgcgtcgg
      121 cgaatcagga gatcggctca ctcgagcggc aaaggtgttg gagcagctga cgggtcaaac
      181 tcctgtcctg tcaaaggcac gctacaccgt ccgttccttc ggaattcgcc gtaacgaaaa
      241 aattgctgtc cactgcaccg ttcgtgggcc aaaagccgaa gagatcatcg accgcggatt
      301 gaaggtgaaa gaatacgagc tgtatcgcga gaacttttct gacactggaa acttcggttt
      361 tggcatccaa gagcacattg accttgggat caaatacgat ccgtcgatcg gaatatacgg
      421 catggacttc tatgttgtgc tgaaccgcgc tggcgttaga gttgccaaac gccgacgcgc
      481 acagggccgc gttggagcct cgcaccgagt gcacaaggag gaagctgtca agtggttcca
      541 acagaagtac gatggagtca ttcttccgcc gaaaccgaag gaagctcgca agggaggacg
      601 tagcaagaga cgctaaacga ttgattggtg aatcgcatgt cctttggatt ttgaacattg
      661 ttgttgattg atttgataaa ttttgtt
//