LOCUS       CO961044                 530 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16e07.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains TAR1.b1 TAR1 repetitive element, mRNA
            sequence.
ACCESSION   CO961044
VERSION     CO961044.1  GI:51334228
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgctagcttt gaggagttcc agaaagtgaa gcaaaactac ttctacggga aggtgcccgt
       61 gctggaggtg gacggcaaac ccttggcgca gagcaccacc attttgctgc tacttgggcg
      121 aaaaattcgg tctggccggc aaggacgagt gggagcgcgc caaggccaac gagatcatcg
      181 acttccacaa ggacgtggtg gccgagttgg cgccctatat ctacgcaaag atgggctatc
      241 gcgagggcaa tgtgccacaa cttcgcaccg acgtgttcgt gcctggacgg gcgcgcttgg
      301 cgcccctcta cgagaagctg ctggccgcat ccggctccgg cttcctgttg gccagcggcg
      361 tgagcatggt cgacttcctg gtggcggact acgtgctcac tctgcgcaag gaggagccgg
      421 aggcgatcgg cgcccaccaa gcgctggtgg agcactccaa gcgtgtgctg gcactcccac
      481 agctccagaa atacgtcagc actcgcaaag agtgagactg tgagacactc
//