LOCUS       CO961041                 554 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16e03.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961041
VERSION     CO961041.1  GI:51334225
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer
            High quality sequence stop: 545.
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttcatttgcc ccacatcaaa gttctctctc tctctctcag cgcccattta ttagaaagca
       61 atacatccga accgagcatg ggcgtgaaca aagagctgct ccaaacacaa gtgcaggcgc
      121 taaagctaac caaacaaact ggtggccact actataaaaa gttgttctcc caacactcgg
      181 atgtgtgcga ctcgtacggg ggcattgacc cggacgaggt gcccaaatcg gccaggtaca
      241 tcatgatggc caacaacgaa ctgcaggcct tcatgcaatt gccgaagaac ttcaacgagg
      301 aacgcgactg gcgctcggcc ctgtccaact tcaaggagca ctacagcgac agcgacgtcc
      361 cgctcaaact ctttaacaaa acaaaggagc cgttcttggc gttgctgcaa aagcacgcgg
      421 ggggaatgag tgctgagcag aaaaagggct gggaggagct gctggacaag gcttacgccg
      481 acttgaaaaa gtacggatgg atctgagtgg aactggagac agccgaaaat gaatacaatg
      541 atatctcttt cttg
//