LOCUS       CO961039                 605 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16e01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961039
VERSION     CO961039.1  GI:51334223
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 654
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgtgtcgacc acgctggacg agattgccaa ggacagcgag cacggcctgg gcgcggtgca
       61 tgccgccgat gacgcgtacg cggtcgcttc ggaagtgtgc cagagcacgg ctgctgcgct
      121 tggcaaacgc atctgggcag tggccgaggg cgcgatggag aagtacgcgc gcgagaagtg
      181 cggcgtcgac ctggactcga aacagtgcac cccgttccag ttctgcgaga cgctgctgcc
      241 caaaaatttc cccaaaggag attccgaggg ggacgtgtgg agtatggatt gcttctgctc
      301 gatgacaatg caatgcagaa ccggcgacta tgacatgatc ggttttgtgc cggtgggtgt
      361 cggcacctac gagatctaca tgaagcgagt ggaccaaatg gtcgcgaaac tggacgccat
      421 gccggccctc agcgaggcag aactgggcca gcatttggag gcgatcaagg acgcgagaaa
      481 gcagccatgg gactggagaa agtaaacgga tgtcccgaga ccacatttga tcagataaat
      541 gcaaatcaat ggtatctcta tcgtctgttg agttcgattt tattcgacat aaagaaatgg
      601 cgtat
//