LOCUS       CO961038                 661 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16d12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961038
VERSION     CO961038.1  GI:51334222
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 662
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gttgcgcata tcgcatttca tttgccgcac atcaaagttc tctctctctc tctcagcgcg
       61 catttgttga aaacgaatag atccgaaccg agcatgggcg tgaacacgga gctgctccaa
      121 acacaggtgc aggcgctaaa gctaagcaaa gagactggtg gcgactacta taaagagttg
      181 ttcgcccaac actcggatgt gtgcgacgcg tacgggggca ttgacccgga cgcggtgccc
      241 aaatcggcca ggtacatcat gatggccaac aacgaactgc aggccttcat gcaattgccg
      301 aagaacttca acgaggaacg cgactggcgc tcggccctgt ccaacttcaa ggagcactac
      361 agcgacagcg acgtcccgct caaactcttt aacaaaacaa aggagccgtt cttggcgttg
      421 ctgcaaaagc acgcgggggg aatgagtgct gagcagaaaa agggctggga ggagctgctg
      481 gacaaggctt acgccgacat gaaaaagtac ggatggatct gagtggaact ggagacagcc
      541 gaaaatgaat acaatgatat cgctttcttg agtagtggca aagcaattca tcctcggaca
      601 gattgatttg ttcaattcct tgacgaataa atttatgcgc ttaaaaaaaa aaaaaaacaa
      661 a
//