LOCUS       CO961034                 437 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16d08.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:RL34_SCHPO O42846 60S RIBOSOMAL PROTEIN L34. [1],
            mRNA sequence.
ACCESSION   CO961034
VERSION     CO961034.1  GI:51334218
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 441
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gcttagaaaa atggccgcgg cgtttgacct atcgcaggcg actctgctac aacacaaagt
       61 cgaacaagac caaggtggtc aagacaccag gaggccgcaa tgtgttacac tatcgcaata
      121 agctcggctc cgtgcccaag tgtggcgaca ctggcgtccc gctgaaagga atccaagctg
      181 cgcgacctcg ccaattggcc aagctgacta agcgaaagaa gaccgtcacg cgtgcatacg
      241 gaggatgcct gagcgctgca gcagtgcgcc agcgaataat ccgcgcattc ttggtcgagg
      301 aacagcagat tgcgaacaag atcctgaaag ccaagagtgc tggaaggaag taaaccgatg
      361 gaaggttttg gttccaccat gttctatttg ttgttgcatt aaagtttgtt ctgaaaaaaa
      421 aaaaaaaaaa aaacaaa
//