LOCUS       CO961032                 539 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16d06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to SW:YO85_CAEEL P34621 HYPOTHETICAL 35.7 KD PROTEIN
            ZK1236.5 IN CHROMOSOME III. [1], mRNA sequence.
ACCESSION   CO961032
VERSION     CO961032.1  GI:51334216
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 566
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gtacaaggtg aacctgaacg agaagcactg gaaccggcgc gtctgggaga agggctacgt
       61 cggaccgcca ctgcccaaga taaagaggac tggagtgccg ccgattccgg tcactgaggg
      121 tgctgtgact ggtgtgcagc gcgcactgat gcgcgagtgg aatgtgatgc agtggctcgc
      181 caacccgtat ctcaacgagg aaatggagcg tccatatctg gagcgctacg gcgacatcat
      241 tgacgagtgg cgccagcgcg aaattgaggc gaaacagcga aaaatgcctt caaagccgaa
      301 gtttacgcaa gtggtgaacg gcgaagtgga gaagcggcgc gccaatgtcg gcaacttgtt
      361 gcattcccat cgaaccgtag aggaccaaat gaaggcgttg atccggcgca acagatggga
      421 ctgactgctg ttggttgttt agctttttca attgaaattt tcacatttgg cgaggcatat
      481 aaaatttaaa atgcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaacaaa
//