LOCUS       CO961031                 695 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16d05.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to contains element MSR1 repetitive element, mRNA
            sequence.
ACCESSION   CO961031
VERSION     CO961031.1  GI:51334215
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tctgccgcgc ccattcccag ccgaccacca catcacatca tccattatgc gcttcctggt
       61 gctcgtcccc ctcggcttgt tgctgcttgc cctctgttgt ctgggtgcga aggagggccg
      121 ctcggactcg tcggagatgc gcgaggggat gtggggcatg gccggctcgg aggaggtgaa
      181 gaagcaggag aaggcgaaca aggagaagca ctcttccgag gagctgggcg gcggacggcc
      241 tcgtccccac gcgctgatga gggcggtagt gcgccgtcgc cgttgaaaat ggggagaaag
      301 aactgtgtat taattagact ggaggggaac tatcgaaagg gccctagaag agcaacatcg
      361 gccctctggt ccagtgtgtt ccctattttc gaataaatat gtattccgcc aaaaaaaaaa
      421 aaaaaaaaaa aaaaaaaaaa gaagaagaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag
      481 aaaaggaata atataagaaa aaaaaaaaaa agaaaaaaaa ccccaacccc caaaattttt
      541 ccccccctgg gggaaatttt cgaaaattcc cctcactggg gggcccccct aaattttctt
      601 ttagggggcc ctttcccccc taaagggggc ttattaaaat ctccgggggc ccctttttta
      661 accccccggg ggggggaaaa cccccggggt ttccc
//