LOCUS       CO961030                 605 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16d04.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to WP:CE25630 Y94H6A.C ;contains OFR.t2 MSR1 repetitive
            element, mRNA sequence.
ACCESSION   CO961030
VERSION     CO961030.1  GI:51334214
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tggacaggtg gtccgggtgg cgaacatgat caagcggatt gggggcttac gggcggcact
       61 caagcagcgc tacttgaccg atgacaaccg ggcaggcgaa tttgtcgggg aagaccagtt
      121 cgggaatcgc tacttccagg agaacaccta cttcatgccg agaaaccgct acgtgatcta
      181 ctccgagagg gtttggctcg actacgacgc gtcccaaatt ccggcggagt ggcacggctg
      241 gctgcaccac atttgcgacg acccgcccac ggtgaagccg cccgttcggc acaagtggat
      301 gatgcagcac cgggagaaca actccctggt tctgggggag aagtacgtgc cctactcgac
      361 cacacgaccg aaaatccagg gatgggtgcc gggtcaggga aaggcatccg aagtgtgagc
      421 tggcaagaaa acaatataga ggagcatggc caattcggcg tgtacttcgt agatgaatgt
      481 aaaaataaaa tgctttaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ccaaaacccc gaaactattt ccctcccagg
      601 ggcaa
//