LOCUS       CO961028                 535 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16d02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9XWV7 Q9XWV7 Y37D8A.15 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO961028
VERSION     CO961028.1  GI:51334212
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gctctcctca ctcaacaaat gaagtcgtcc gtcctcgttt ccgctctgct gtgcgccatc
       61 ccctcacttc tgctccgagt ccaggccaac gcggcgcccg atgcggccag cgactttcta
      121 gtgccggtgc cgtcagtgtg tgcacagctg gccggaagtg acgaagagcg aatgctgttg
      181 tgccaactgt acgagtcgtc agccctgctg gcgcaattag gtgctcttgt gaacgaggga
      241 atcggccgac tggcgaccag ccaaggattg gccagagccc cgtcagttgc agcggcggac
      301 gctggccgag agaagcgaaa gcacgagtac ttgcgcttcg gcaagcgaaa gcacgagtat
      361 ctgaggttcg gcagaaagtg aggcagaggg cacaaagaag gtggaaggaa ctggcaaagc
      421 gagaaaacat cacatcccct ccggaactca accggtcgat tgattcgcag atttttgttt
      481 ggaacagcga tctctctcag catcggatga atgcagctga caaaaaaaaa aaaaa
//