LOCUS       CO961023                 391 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16c09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:P92022 P92022 T10G3.6 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961023
VERSION     CO961023.1  GI:51334207
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 395
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttcttctctt ttttcagtca atgggtgggg agggaagtgg tgattgagtt gaagaatgac
       61 ctcagcattt gcgggacatt gcattctgtc gaccaatatc tgaacatgaa gttgtccgac
      121 atatctgttg cagacataga aaggcacccg catatgattt ccgtaaagaa ctgcttcata
      181 cgcggctcag tcattcgcta tgcgcacttg cctgtggatg gcatcgatac tgcactcctg
      241 caggaagcgt cgagaaagga aattttgcag agtcgtcaac aacaagcagc caaatagagt
      301 ggaataaatt tacaaacatt ttacaatgtt ttgatctccc accttgttgc gaacggaaac
      361 aacatttaaa gcaaaaaaaa aaaaaaacaa a
//