LOCUS       CO961022                 370 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16c06.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O01542 O01542 CODED FOR BY C. ELEGANS CDNA
            YK66C9.5. [1], mRNA sequence.
ACCESSION   CO961022
VERSION     CO961022.1  GI:51334206
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 622
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ctccagtatg tccaccagcg gctcaaaaga caacttcatt gcgcaactga aggacggtgt
       61 gagactctgc aacctggcca acaaactgga ggcaaacgct gtgagcggcg ttcacgccaa
      121 ggcgaactcc agcttccaat gcatgaacaa tttggagctg ttcctgagct ttgccaaacg
      181 ccagggcgtc atcacgcagg agctgttccg cgctgtcgat ttggttgagg cgcgtgacct
      241 ctactcagtt tgcatgacac tcaattcgct tggacgcatt ctggcaaagc aaggcaagcc
      301 tcatccagga catgtgaatg ccactgaaat tttgaacatc ccgtccggtg atgccctccg
      361 tcttcactga
//