LOCUS       CO961018                 723 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16c02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q9TYS3 Q9TYS3 D1037.3 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961018
VERSION     CO961018.1  GI:51334202
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 779
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 tgcttttgcc gattccccaa atggcacaca cccccacttc gctgattccc ctgaactacg
       61 cggccgagtc ggaggccggg gtgaacaagc agatcaacat ccagctgtac accagctacg
      121 tctacttggg catgtcctac tattttgacc gcgaggatgt tgctctgcac aacatctcaa
      181 aatacttcaa aaagctgtcc gacgaggagc gggaggaagc ggccaaactg atgaagtacc
      241 agaacactcg cggcggacga atcattttga agaacatcga gaagccggag aaagacgaat
      301 ggggcacccc tctggaggcg ttcaaatccg cactggcgtt gcaaaagtac aacaaccaga
      361 cgctgctgga gctgcacaaa attgccgaca aaaacgacga ccccgagatg cacgacttca
      421 tcgaggagga gtttctccgc aagcaggtcc agctgatcga acagctcggc aaggcgatcg
      481 ccaatctgca acgcgtgggc gaggggctcg gcgagtactt gttcgacaag gagcagttcg
      541 aggactgagt gctagaggaa cggacacaga gacagcacag acgggaaaag agtgccgctt
      601 ctgagagtaa cgctgacagt cacttacaca cttagccagg cgaagaattg ttaaaagttc
      661 cactgaagtg gttgttgcga tcaccccgta atttcaatcg agaaaataag aaataacgaa
      721 caa
//