LOCUS       CO961017                 748 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16c01.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:Q65358 Q65358 HYPOTHETICAL PROTEIN ;contains
            element TAR1 repetitive element, mRNA sequence.
ACCESSION   CO961017
VERSION     CO961017.1  GI:51334201
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Possible reversed clone: similarity on wrong strand
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ggcgttgcgc attcatcctg ctcttggtcg tcatcggtgt gcttgccatg ctggagctca
       61 ccactgccga tccaatcact cacttcaatg agcgcgtggt cgggctgctg gtggagcacg
      121 aggcggacga gccgatgatt gagcgcatga ggcgctgggg cggcggcggc atcggagcgg
      181 agcgaatgcg acgccaaatg ggcggtggag ccggtggtgg tggcggaggc ggcagaaact
      241 gttgcgactg ctgcactggc tcttgcggat gcgagatttg ctgtgtaccg aactggggcc
      301 gcaaatgaag gaacggagat tgggaacaat aaacagtcga tcaatagaca aattgatatg
      361 ccatttatgc ggtgaatgga ctgaaattgc gtacaaattt gaattccaaa aaaaaaaaaa
      421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa accgggaccc ctgaattttt ttccccccag
      541 ggggaaattt cgcaaaattc cctccaaggg ggggcccccc gaccttggtt ttagggggcc
      601 cctttccccc taaagggggt ttatttaaat ttccgggggc gtttttttac aactccgggg
      661 gggggaaacc cctgggtttc cccccttttt ccccctgggc ccaatccccc ctttcccccc
      721 cgggggaaaa aacaaaaagg cccccccc
//