LOCUS       CO961014                 504 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16b09.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5', mRNA sequence.
ACCESSION   CO961014
VERSION     CO961014.1  GI:51334198
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 597
            Seq primer: SL1 primer
            High quality sequence stop: 501.
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 cctaaatcgc tgtgcattta cgaccgtgct gaagcaacca ttttccactt caacaccagc
       61 gttcaatcca ttccgttttg gctttaaaaa atggaatatt ttcgcaccca aacgcttaaa
      121 ctggatgcca cagaacactc tggaacggaa attgttcgaa gaacagaaga aaaatgaatt
      181 tttgacactg caacttttgc tcgaaaaatc gagtatgaaa attctcagca ctgatatggg
      241 catccaaatg caatacgccg ctgcccaaag tgccgttcag acaattggct atgagtcgtg
      301 cagtggcatg gcagcaaatg ttgtgaagaa ttttgcgaag caactggatc tggaccgcaa
      361 ccaatggaac tgttttgtct ttgatactga ttaccgtaca aaatgcgatt ttgtacatac
      421 gagcaagttc accgactaca ttttgttctc atacaggcgc ttcaatattg tcctcttcaa
      481 agagcactga tttgaatcgt aatg
//