LOCUS       CO961009                 544 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16b02.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O62289 O62289 JC8.8 PROTEIN. [1], mRNA sequence.
ACCESSION   CO961009
VERSION     CO961009.1  GI:51334193
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Putative full length read
            The vector to vector length is 587
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 gttgtttgcg gtggtgctct tcgccataat caccattgtg ccgtccccgt gcggagcaat
       61 gcgccaacag gcggtcgcag tcaagggcca gctgatgtgc gggtcgaggc ccgcctcggg
      121 ggtgaaggtg aagctctggg acgaggacga cggcccagac ccggacgacg tgctggacga
      181 ggcgttcacc gactccagcg gcgcattcca cctgaagggc tccgagcgcg agctgaccaa
      241 cattgacccg gtgctcaaaa tctaccacga ttgcgacgac gggattatgc caggacagcg
      301 caagatcaaa ctgcgcattc ctggctctta catcacgggc ggaggcgtcg caaaacgcgt
      361 gttcgacgtt ggattgctca atttggagac caaattcccg ggcgaggagc gagatttgct
      421 ctgatggaag tgatcagacg aggaaaacac aacatttttc taaatttcaa ttgaatttag
      481 attcacaaat tttaaaaatt gctgcattca aatttcaaaa gtgcgcgctc aaacagcaaa
      541 aaaa
//