LOCUS       CO961007                 664 bp    mRNA    linear   EST 12-JAN-2011
DEFINITION  pe16a12.y1 Radopholus similis SL1 TOPO v1 Radopholus similis cDNA
            5' similar to TR:O44738 O44738 F57B10.5 PROTEIN. [1], mRNA
            sequence.
ACCESSION   CO961007
VERSION     CO961007.1  GI:51334191
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   McCarter,J., Clifton,S., Chiapelli,B., Pape,D., Martin,J.,
            Wylie,T., Dante,M., Marra,M., Hillier,L., Kucaba,T., Theising,B.,
            Bowers,Y., Gibbons,M., Ritter,E., Bennett,J., Franklin,C.,
            Tsagareishvili,R., Ronko,I., Kennedy,S., Maguire,L., Beck,C.,
            Underwood,K., Steptoe,M., Allen,M., Person,B., Swaller,T.,
            Harvey,N., Schurk,R., Kohn,S., Shin,T., Jackson,Y., Cardenas,M.,
            McCann,R., Waterston,R. and Wilson,R.
  TITLE     The Washington Univ. Nematode EST Project, 1999
  JOURNAL   Unpublished
COMMENT     Contact: Mitreva M
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library constructed by: Irina Ronko and Dr. Makedonka Mitreva
            Seq primer: SL1 primer.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_016211 Radopholus similis SL1 TOPO v1"
                     /dev_stage="Mixed"
                     /lab_host="GC10"
                     /note="Vector: pCRII-TOPO (Invitrogen); Site_1: 5': EcoRI;
                     Site_2: 3': EcoRI; The library was constructed by Irina
                     Ronko and Dr. Makedonka Mitreva
                     (mmitreva@watson.wustl.edu) at Washington University, St.
                     Louis. Oligo(dT)-SL1 PCR based library. Radopholus similis
                     cDNA PCR products of size >400 nucleotides containing SL1
                     on the 5' end and oligo(dT) on the 3' end were
                     non-directionally cloned into pCRII-TOPO(Invitrogen)
                     following the Topo TA cloning protocol. Radopholus similis
                     total RNA was provided by Dr. Godelieve Gheysen of Ghent
                     University (lighe@gengenp.rug.ac.be)."
ORIGIN      
        1 ttccgccgaa caactgctgc tgttcgctct gctctacttg gaactgtacg cgtatgcttc
       61 tgagctcaca ttcgaactac ctgacaacgc caatcagtgc ttttacgaag agatgaagaa
      121 aggcattgac tgtacacttg agttccaggt tgtcactggt gggcaatatg atgtggatgt
      181 ggttttggag gacccaaatg gtcgagttat gtataaggag gcccggaaac aattggacag
      241 tcacccgtac aagaccgaag ttgacggaac ctacaaagtg tgtttttcga acgagttctc
      301 aaccttttcg cataaagttg tttacatgga ctggcaagtg ggagctcctg aattcactgg
      361 attgcctact ccacgcgttg gaggtggaac ttcacagatg gaacgcagtg ttgagacgat
      421 agctgatcga ctcaggattg tggacgacta tcaaacacat catcgtttgc gcgaagcaac
      481 tggccgaaag agagcagagg atttaaatga acgcgtgacc tactggtcct tggggcaaac
      541 agcggtcgtg gtcataatcg gtattggaca ggtgttcttg ctgagatctt ttttttcgga
      601 cagaaaacca gctggatact aatggatgat caattctatt ggtggataaa ttaatttgct
      661 tgtc
//